Cloned (Comment) | Organism |
---|---|
expression in Rosetta2 cells and in COS-7 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D35A/K196A | reduced specificity for shorter peptides | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
calcitonin gene-related peptide | - |
Mus musculus | |
S17092 | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
calcitonin gene-related peptide | mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide | Mus musculus | |
0.0034 | - |
calcitonin gene-related peptide | mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide | Mus musculus | |
0.0043 | - |
calcitonin gene-related peptide | wild type enzyme, full length calcitonin gene-related peptide | Mus musculus | |
0.01 | - |
calcitonin gene-related peptide | wild type enzyme, shorter peptide of calcitonin gene-related peptide | Mus musculus | |
0.0125 | - |
calcitonin gene-related peptide | wild type enzyme, diserine mutant of calcitonin gene-related peptide | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calcitonin gene-related peptide + H2O | assay at pH 7.0, 37°C | Mus musculus | ? | - |
? | |
Substance P + H2O | - |
Mus musculus | ? | - |
? | |
Z-Gly-Pro-7-amido-4-methylcoumarin + H2O | assay at pH 7.0, 37°C | Mus musculus | z-Gly-Pro + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PREP | - |
Mus musculus |
prolyl endopeptidase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
calcitonin gene-related peptide | wild type enzyme, full length calcitonin gene-related peptide | Mus musculus | |
0.002 | - |
calcitonin gene-related peptide | wild type enzyme, diserine mutant of calcitonin gene-related peptide | Mus musculus | |
0.0023 | - |
calcitonin gene-related peptide | mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide | Mus musculus | |
0.0051 | - |
calcitonin gene-related peptide | mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide | Mus musculus | |
0.072 | - |
calcitonin gene-related peptide | wild type enzyme, shorter peptide of calcitonin gene-related peptide | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
- |
Mus musculus | calcitonin gene-related peptide |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
calcitonin gene-related peptide | wild type enzyme, diserine mutant of calcitonin gene-related peptide | Mus musculus | |
0.22 | - |
calcitonin gene-related peptide | mutant D35A/K196A, full length calcitonin gene-related peptide | Mus musculus | |
0.28 | - |
calcitonin gene-related peptide | wild type enzyme, full length calcitonin gene-related peptide | Mus musculus | |
0.66 | - |
calcitonin gene-related peptide | mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide | Mus musculus | |
4.3 | - |
calcitonin gene-related peptide | mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide | Mus musculus | |
6.9 | - |
calcitonin gene-related peptide | wild type enzyme, shorter peptide of calcitonin gene-related peptide | Mus musculus |